Lipopolysaccharide-binding protein inhibitory peptide inhibits the binding of LPS to U937 cells
- VernacularTitle:脂多糖结合蛋白抑制肽抑制脂多糖与人单核巨噬细胞株的结合
- Author:
Xueling WU
;
Guisheng QIAN
;
Yunfeng ZHAO
;
Debin XU
;
Weizhong CHEN
- Publication Type:Journal Article
- Keywords:
U937 cells;
Lipopolysaccharide-binding protein inhibitory peptide;
Lipopolysaccharides
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the inhibitory effect of P12,a kind of lipopolysaccharide(LPS)-binding protein(LBP) inhibitory peptide,on the binding of LPS to macrophage in vitro.METHODS:Human monocyte-like cell line(U937 cells) was grown in RPMI-1640 and stimulated with PMA in order to induce their differentiation to macrophage stage.The relative affinity of P12 to LPS was determined by enzyme-linked immunosorbent assay(ELISA).The effects of P12 on the binding of LPS to U937 cells were determined by flow cytometry analysis.The production of tumor necrosis factor-alpha(TNF-?) was measured by ELISA.RESULTS:The relative binding activity of P12 to LPS was higher than that of LBP in the same mass concentration.P12 inhibited the binding of FITC-conjugated LPS(FITC-LPS) to U937 cells.The productions of TNF-? was also significantly suppressed by P12.CONCLUSION:The results suggest that blockage of LBP at the inflammatory sites might attenuate LPS-induced circulatory shock.
