Nuclear factor ?B in LPS stimulated rat alveolar macrophages promotes TNF-? secretion
- VernacularTitle:LPS致大鼠肺泡巨噬细胞NF-?B促进TNF-?分泌
- Author:
Fang ZHANG
;
Ziling LI
;
Yi SHI
;
Ming ZHAO
;
Xiaofeng XIN
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
Macrophages,alveolar;
NF-kappa B;
Lipopolysaccharides;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the activation of nuclear factor ?B(NF-?B) induced by lipopolysaccharides(LPS) in rat alveolar macrophages(AMs) and its regulatory role in tumor necrosis factor(TNF-?) secretion.METHODS:The dynamic activity changes of NF-?B induced by LPS were determined with electrophoretic mobility shift assay(EMSA).Antisense oligonucleotides of NF-?B subunit(p65) were transfected into AMs prior to LPS stimulation.The effect of antisense oligonucleotide transfection on expressions of p65 and TNF-? in supernatant were measured with Western blotting and enzyme linked immunosorbent assay(ELISA),respectively.RESULTS:NF-?B activity increased markedly and reached its peak level at 4 h after LPS stimulation.After transfected with antisense oligonucleotides of NF-?B subunit(p65),expression of p65 and TNF-? in supernatant decreased markedly.CONCLUSION:NF-?B activity has a positive effect on regulating secretion of TNF-? in AMs induced by LPS.
