Effects of FKBP12.6 overexpression on the performance of sarcoplasmic reticulum in ventricular myocytes of rats with heart failure
- VernacularTitle:钙释放通道稳定蛋白过度表达对心力衰竭心室肌细胞肌浆网功能的影响
- Author:
De LI
;
Wei WU
;
Ning LUO
;
Shuxian ZHOU
;
Chang FANG
- Publication Type:Journal Article
- Keywords:
FKBP12.6;
Sarcoplasmic reticulum;
Gene transfer,horizontal;
Heart failure,congestive
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effects of FK506 binding protein 12.6(FKBP12.6) overexpression on the performance of sarcoplasmic reticulum(SR) in ventricular myocytes of rats with heart failure.METHODS:The adenovirus(Ad)-mediated gene transfer was used to overexpress FKBP12.6 in ventricular myocytes of rats with heart failure.Western blotting and reverse transcriptase-polymerase chain reaction(RT-PCR) analysis were used to reveale specific overexpression of FKBP12.6.X-rhod-1-AM was used as the Ca2+ indicator,and cells were viewed under a confocal microscope.RESULTS:Adenovirus mediated overexpression of FKBP12.6 resulted in a 5-fold increase in relative FKBP12.6 mRNA levels and a 4-fold increase in relative FKBP12.6 protein levels at 48 h after transfection compared with control.The amplitude of the fluorescence [Ca2+]i transient was significantly increased in Ad-FKBP12.6-GFP cardiomyocytes compared with Ad-GFP myocytes(peak F/F0,3.16?0.42 vs 1.43?0.38,P
