Effect of vitamin K_3 on apoptosis induced by androgen-independent prostate cancer cell PC-3M
- VernacularTitle:维生素K_3对雄激素非依赖性前列腺癌PC-3M细胞的凋亡诱导作用
- Author:
Lei ZHOU
;
Yang LI
;
Jing SU
;
Jinsong KANG
;
Guifang MU
;
Zhi WANG
;
Li XU
;
Xuejian ZHAO
;
Liankun SUN
- Publication Type:Journal Article
- Keywords:
Vitamin K_3;
Prostatic neoplasms;
Apoptosis;
PC-3M cells
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the effect of vitamin K3(VK3) on the induction of apoptosis in androgen-independent prostate cancer cell PC-3M in vitro.METHODS:Cell viability was estimated by MTT assay.AO/EB staining was performed to detect apoptotic cells.Apoptosis and the changes of cell cycle were detected by flow cytometry.NAC was used to observe the effect of growth inhibition by VK3.RT-PCR was used to confirm the changes in gene expression.Levels of intracellular peroxides were estimated by using an oxidation-sensitive fluorescent probe DCFH-DA.RESULTS:PC-3M cells growth was significantly inhibited by VK3(≥60 ?mol/L,P
