Induction of apoptosis by c-myc antisense oligonucleotide in osteosarcoma cell MG-63
- VernacularTitle:反义c-myc寡核苷酸诱导骨肉瘤细胞MG-63凋亡的实验研究
- Author:
Jun HU
;
Guilong WANG
;
Yang XIANG
;
Yi LIU
;
Yisheng CHEN
;
Ruhu DING
- Publication Type:Journal Article
- Keywords:
Genes,c-myc;
Oligonucleotides,antisense;
Osteosarcoma;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the induction of apoptosis by c-myc antisense oligonucleotide in osteosarcoma cell(MG-63).METHODS:The designed c-myc antisense oligonucleotide fragment was transfected into human osteosarcoma MG-63 cells.The cell growth and apoptosis were measured by the methods of MTT,FCM,HE staining and transmission electron microscopy.RESULTS:The results showed that the proliferation of human osteosarcoma MG-63 cells was inhibited and apoptotic rate was 37.92% when treated with c-myc antisense oligonucleotide at the does of 10.0 ?mol/L for 48 h.c-myc antisense oligonucleotide(10.0 ?mol/L) also inhibited the expression of c-myc protein.CONCLUSION:c-myc antisense oligonucleotide is able to induce apoptosis in human osteosarcoma MG-63 cells.
