Effect of antisense RNA targeting Polo-like kinase 1 on cell growth in A549 lung cancer cells
- VernacularTitle:Polo-like激酶l反义RNA对肺癌细胞A549生长的实验研究
- Author:
Qiong ZHOU
;
Jianchu ZHANG
;
Yang JIN
;
Xiaoju ZHANG
;
Xiaonan TAO
;
Ming BAI
- Publication Type:Journal Article
- Keywords:
Polo-like kinase-1;
RNA,antisense;
Cell cycle;
Lung neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Polo-like kinase-1(Plk1) depletion on cell cycle progression and cell growth in lung cancer cells.METHODS:A recombinant plasmid containing antisense RNA targeting Plk1(pcDNA3-Plk1) was transfected into A549 cells by lipofectine.RT-PCR and Western blotting were used to examine Plk1 gene expression.Cell proliferation was evaluated by cell counting and BrdU labeling.Cell cycle distribution and apoptosis were examined by flow cytometry.Inhibition rate(IR) of vinorebline(NVB) was determined by MTT assay.RESULTS:After transfected with pcDNA3-Plk1 into A549 cells,the expression levels of Plk1 mRNA and protein were greatly decreased.Abnormal morphological changes of cells and growth inhibition were observed in pcDNA3-Plk1 transfected cells.The BrdU labeling index was significantly lower than that in control group(P
