An experimental study on time-dependent degradation of DNA in lymphocytes in the human bloodstain by single-cell gel electrophoresis
- VernacularTitle:应用单细胞凝胶电泳测定人血痕淋巴细胞降解的实验研究
- Author:
Jilong ZHENG
;
Xiaona LI
;
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Forensic pathology;
Single cell gel electrophoresis;
Aging of bloodstain;
lymphocytes
- From:
Chinese Journal of Forensic Medicine
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study time-dependent degradation of DNA in lymphocytes in the human bloodstain in order to estimate aging of bloodstain.Methods Lymphocytes were isolated from the human bloodstain placed under room temperature and were analyzed by single-cell gel electrophoresis(SCGE).The percentage of DNA-degraded lymphocytes were calculated in the cloodstain placed at different time intervals from 0 to 72h under fluorescent microscope connected with auto-analysis-image system.Results The percentage of DNA degraded lymphocytes was closely correlated with time lapse within 72h.A regression equation was formulated with R=0.9522(P
