Determination of Scutellarin in Human Plasma by HPLC-ECD
- VernacularTitle:库仑阵列电化学HPLC法测定人血浆中灯盏乙素的浓度
- Author:
Jingli DUAN
;
Suodi ZHAI
- Publication Type:Journal Article
- Keywords:
HPLC-ECD;
Scutellarin;
Pharmacokinetics
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a HPLC-ECD method for the determination of scutellarin in human plasma.METH-ODS:The plasma sample injection was performed after protein precipitation by methanol.The mobile phase consisted of 0.083mol? L-1 sodium dihydrogen phosphate-methanol-acetonitrile-tetrahydrofuran(60:30:10:0.4),which was added dropwise with H3PO4 in a ratio of 100:106).The column temperature was under room temperature;the detection voltage was 100mV;the protection voltage was 500mV;the flow rate was 1mL? min-1 and the sample size was 20? L.RESULTS:The calib-ration curve was linear in the range of 20.0～ 400.0?g? L-1(r=0.999 9).The methodological recovery was 96.79% ～ 108.50%.Both the intra-day and inter-day RSD were less than 5%.CONCLUSION:This HPLC-ECD method is accurate,sensitive and simple,and suitable for pharmacokinetic study of scutellarin.
