In Vitro Induction and Differentiation of Rabbit Bone Mesenchymal Stem Cells into Cardiomyocytes
- VernacularTitle:兔骨髓间充质干细胞体外定向诱导分化为心肌细胞的实验研究
- Author:
Lixing CHEN
;
Jun NIE
;
Ying XU
;
Jianming XIAO
;
Hongyan CAI
;
Min ZHANG
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Induced differentiation;
5-azacytidine
- From:
Journal of Kunming Medical University
1988;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To further prove the multipotentiality of rabbit bone mesenchymal stem cells (MSCs) by inducing it into cardiomyocytes with 5-azacytidine. Methods MSCs from rabbit were abstracted, isolated, cultured. Then MSCs were induced by 5-azacytidine in vitro for 24 hours. After cultured for 4 weeks, MSCs’ differentiation was studied by immunohistochemistry and electron microscopy technique. Results Some of MSCs induced by 5-azacytidine expressed troponinT . Electron microscopy showed myofilaments. Conclusion MSCs which exist in bone marrow are mutipotential stem cells, and they can be induced into cardiomyocytes by 5-azacytidine in vitro.
