Roles of Rho-kinase in rat cardiac fibroblasts proliferation and collagen synthesis induced by angiotensin Ⅱ
- VernacularTitle:Rho激酶在血管紧张素Ⅱ刺激大鼠心肌成纤维细胞增殖和胶原合成中的作用
- Author:
Xianghai WANG
;
Wei WU
;
Jun YANG
;
Chang FANG
;
Dengfeng GENG
;
Zhibin HUANG
- Publication Type:Journal Article
- Keywords:
Cardiac fibroblasts;
Rho-kinase;
AngiotensinⅡ
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of Rho-kinase signal pathway in rat cardiac fibroblasts (CFBs) proliferation and collagen synthesis induced by angiotensinⅡ (AngⅡ). METHODS: CFBs of neonatal Sprague-Dawley (SD) rats were isolated with the method of trypsin digestion and differential anchoring velocity. The CFBs were stimulated with AngⅡto induce fibrosis. Proliferation of CFBs was observed by MTT coloricmetric assay. Synthesis of collagen was detected by the hydroxyproline. The expression of Rho-kinase mRNA was examined using RT-PCR analysis. The extent of phosphorylation of myosin-binding subunit (MBS-P) of myosin phosphatase was quantified by Western blotting analysis, which was used to evaluate the activity of Rho-kinase.RESULTS: (1) Stimulation of neonatal SD rat CFBs with AngⅡ (10-7 mol/L) significantly increased CFBs proliferation and collagen synthesis (P
