Effects of dexamethasone on intracellular expression of T_H1/T_H2 cytokines in experimental asthma mice
- VernacularTitle:地塞米松对实验性哮喘小鼠T_H1/T_H2细胞因子的影响及其机制的探讨
- Author:
Shixiu PAN
;
Tianshu ZENG
;
Tianpen CUI
;
Lihua HU
;
Jianmin WU
- Publication Type:Journal Article
- Keywords:
Asthma;
Interleukin-4;
Interferon type Ⅱ;
Dexamethasone;
GATA-3;
T-bet
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of dexamethasone (DXM) on intracellular expression of TH1/TH2 cytokines and the mechanism of that during the development of asthma. METHODS: Eighteen BALB/c mice were divided into 3 groups at random: control group, asthma group, and DXM treated group, with 6 mice in each. The expressions of T-box expressed in T cells (T-bet) and GATA-binding protein-3 (GATA-3) in lung tissue were detected by Western blotting. The expressions of intracellular cytokines interleukin-4 and interferon-? in CD4+ T cell were measured by flowcytometry.RESULTS: The results of flow cytometry indicated that the ratio of intracellular cytokines IL-4/IFN-? in CD4+ T cells in asthma group was much higher than that in control group (P
