Effects of taurine on the change of apoptosis induced by IL-1?, TNF-? and IFN-? in rat pancreatic islet cells
- VernacularTitle:IL-1?、TNF-?和IFN-?引起的大鼠胰岛细胞凋亡及牛磺酸的影响
- Author:
Huarong YU
;
Neng ZHANG
;
Yilong DONG
;
Qin ZHOU
;
Qixin ZHOU
- Publication Type:Journal Article
- Keywords:
Interleukin-1;
Apoptosis;
Nitric oxide;
Gene expression;
Taurine;
Islets of langerhans
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the changes of apoptosis in isolated pancreatic islet cells, insulin secretion, expression of Bcl-xL and Bax induced by combination of IL-1?, TNF-? and IFN-?, and effects of taurine on them. METHODS: Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro. NO-2/ NO-3 production, NOS activity, insulin secretion, the protein expression of Bcl-xL and Bax, percentage of islet cell apoptosis and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1?, TNF-? and IFN-? were measured, and the effects of taurine on the changes of them were further investigated. RESULTS: Combination of IL-1?, TNF-? and IFN-? induced a significant increase in percentage of pancreatic islet cell apoptosis, NO-2/ NO-3 production and NOS activity, DNA ladder appearance, a decrease in insulin content, up-regulation in the protein expression of Bax and down-regulation in the protein expression of Bcl-xL (P
