Study on the immunological function of sodium butyrate-induced immature human monocyte-derived dendritic cells
- VernacularTitle:丁酸钠诱导的未成熟树突状细胞的免疫学功能研究
- Author:
Lu LIU
;
Jun MIN
;
Jie WANG
;
Changzhen SHANG
;
Yunle WAN
;
Qiang YU
;
Mei YANG
;
Jun CAO
;
Jisheng CHEN
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Butyrates;
Immunotolerance;
Interleukin-12
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the immunological function of sodium butyrate-induced immature dendritic cells in vitro.METHODS: The human monocyte-derived dendritic cells were induced in the presence of human granulocyte macrophage-colony stimulating factor(GM-CSF) and interleukin-4 (IL-4), combined with sodium butyrate. The immunological function of sodium butyrate-induced dendritic cells was detected by the FCM, endocytic activity, T cells stimulatory proliferation capacity, and interleukin-12 (IL-12) production.RESULTS: Sodium butyrate could down-regulate the major histocompatibility complex(MHC) class II and costimulatory molecules of dendritic cells, increase the endocytic activity, induce a stage of T-cell anergey, and inhibit the T helper cell type 1-skewing factor IL-12 production. CONCLUSION: Sodium butyrate inhibits the maturation of dendritic cells and induces production of immature dendritic cells, which may help to explore the machenism of its epigenitic modification.
