Expression of HIF-1? in rat's radiation-induced oral mucositis
- VernacularTitle:缺氧诱导因子-1?在大鼠放射性口腔黏膜炎中的表达及意义
- Author:
Chongjin FENG
;
Junbing GUO
;
Hongwei JIANG
;
Chunyang LI
;
Bin CHENG
;
Yong CHEN
;
Hanyu WANG
;
Huijun LI
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor 1?;
Radiation;
Oral mucositis;
Rats
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect the mRNA and protein level of HIF-1 alpha in the tissues of rat's radiated mucosa. METHODS: The left buccal mucosa was irradiated and excised. The right buccal mucosa was excised to serve as own control tissue. The mRNA of HIF-1 alpha was determined by using the semi quantitative RT-PCR. SABC method was employed to immunostain and to elucidate the localization, intensity and distribution of HIF-1 alpha protein.RESULTS: A Sprague-Dawley rat's model of radiation-induced oral mucositis (ROM) was successfully established. The results of RT-PCR indicated that the left buccal mucosa expressed HIF-1 alpha mRNA while the right buccal mucosa did not or seldom expressed it. Immunohistochemical analysis of HIF-1 alpha demonstrated that the left side mucosa expressed HIF-1 alpha protein.CONCLUSION: The mucosa of ROM expresses the mRNA and protein of HIF-1 alpha. The expressions of HIF-1 alpha are correlated with the severity of ROM.
