The effect of antisense mTOR gene mediated by nanoparticles on neointimal formation in vein graft in rats
- VernacularTitle:纳米粒子介导反义mTOR基因转染抑制移植静脉内膜增生
- Author:
Xinhua HU
;
Qiang ZHANG
;
Jun YANG
;
Chengwei LIU
;
Zhishen ZHANG
;
Dehua YANG
- Publication Type:Journal Article
- Keywords:
mTOR;
Intimal Hyperplasia;
Nanoparticle;
Transfection;
Gene Expression
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of antisense mTOR gene transfection mediated by nanoparticles(NP) on intimal perliferation after vein grafting.Methods Nanoparticle antisense mTOR gene complex was prepared with PLGA and PVA.Autogenous vein graft model was established in 72 rats by transplanting internal branch of jugular vein to carotid artery.Three groups were studied:(1) antisense mTOR group,antisense mTOR gene mediated by NP was transfected into the veins before anastomosis.(2) Empty vector group,the vein was transfected by empty vector mediated by NP.(3) Control group,no transfection.The grafted veins were harvested 3 days,1 week,2 weeks and 4 weeks after operation,respectively.The exogenous mTOR mRNA and protein expression were determined and intimal hyperplasia(IH) was observed.The presence of apoptotic VSMC was also detected.Results Antisense mTOR gene transfection mediated by nanoparticle complex inhibited the mRNA and protein expression of mTOR gene(P
