Determination of Puerarin in Compound Ginkgo Biloba Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定复方银杏叶片中葛根素的含量
- Author:
Hongping PAN
;
Hualing WEI
;
Daping MENG
;
Mei LU
- Publication Type:Journal Article
- Keywords:
Compound Ginkgo biloba tablets;
Puerarin;
RP-HPLC;
Content determination
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of Puerarin in Compound Ginkgo biloba tablets by RP-HPLC. METHODS:The determination was performed on Kromasil C18 column. The mobile phase consisted of methanol-water (20∶80) with a flow rate of 1mL?min-1. The detection wavelength was 250 nm and the temperature of the column was 35℃. RESULTS:The linear range of Puerarin was 0.8～2.4?g (r=0.999 3, n=5),with the average recovery at 99.98%,RSD=1.31%(n=5).CONCLUSION:The method is simple,sensitive and reproducible,and it can be used for the quality control of Compound Ginkgo biloba tablets.
