Determination of Demethylbellidifolin in Different Parts of Swertia Davidi Franch. by HPLC
- VernacularTitle:川东獐牙菜不同部位去甲基雏菊叶龙胆酮的含量测定
- Author:
Youli ZHANG
;
Yimin ZHENG
;
Xiuying XU
;
Shanquan FU
- Publication Type:Journal Article
- Keywords:
Swertia davidi Franch.;
Demethylbellidifolin;
HPLC;
Content determination
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a HPLC method for the determination of the content of Demethylbellidifolin in different parts of Swertia davidi Franch. METHODS: The analysis was carried out on Hypersil C18 column (150mm?4.6mm,5 ?m) at room temperature with mobile phase consisted of CH3OH-0.5%H3PO4(56∶44) at a flow-rate of 1.0mL?min-1.The detection wavelength was set at 254 nm. RESULTS: The linear range of Demethylbellidifolin was 0.52～2.60?g (r=0.999 4) and the average recovery was 99.77%(RSD=0.95%).CONCLUSION: The method is simple, rapid, reproducible, and suitable for the determination of the content of Demethylbellidifolin in Swertia davidi Franch..
