Effects of spermine on myocardial ischemia/ reperfusion injury in rats
- VernacularTitle:精胺减轻大鼠在体心肌缺血/再灌注损伤的作用及机制初探
- Author:
Liping HAN
;
Chunming JIANG
;
Hongzhu LI
;
Guan WANG
;
Yihua SUN
;
Changqing XU
- Publication Type:Journal Article
- Keywords:
Spermine;
Myocardial reperfusion injury;
Reactive oxygen species;
Rats
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effect of exogenous spermine (low concentration) on myocardial ischemia/reperfusion injury in rats.METHODS: 40 Wistar rats were randomly divided into 4 groups: sham- operation group (Sham), ischemic reperfusion group (I/R), spermine group (Sp) and natural saline group (NS). The model of ischemic/reperfusion injury was established by ligating rat coronary artery. In Sp group, spermine (0.5 mmol/L, 2 mL/kg) was injected slowly into rat vein. During the process, we recorded the electrocardiogram and the LV functional parameters, assayed the levels of SOD, LDH, NO and MDA in serum, and examined the ultrastructure of the myocardium. RESULTS: In I/R group, the incidence of arrhythmia was 90%, myocardial ultrastructure was injured seriously, values of LVSP and ?dp/dtmax decreased, levels of LDH, NO and MDA increased while SOD activity decreased (P
