Comparative phosphoproteome analysis of cardiomyocytes preconditioned by diazoxide
- VernacularTitle:二氮嗪预处理心肌细胞后磷酸化蛋白质组差异表达的初步研究
- Author:
Hong LI
;
Yingbin XIAO
;
Tiande YANG
;
Zhirong MOU
;
Liyun ZOU
;
He HUANG
- Publication Type:Journal Article
- Keywords:
Proteome;
Cardiomyocytes;
Diazoxide
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To analyze and identify the phosphoproteins associated with diazoxide preconditioning. METHODS: Proteomics technique was used to investigate the changes of phosphoprotein after diazoxide preconditioning. Adult rat ventricular myocytes were pretreated in the presence and absence of 200 ?mol/L diazoxide for 10 min. Phosphoproteins prepared and enriched respectively from control and diazoxide pretreated groups were then separated by two-dimensional (2D) gel electrophoresis and stained with sliver staining kit. Phosphoproteins of interest were further identified by mass spectrometry. RESULTS: Associated with diazoxide preconditioning, the proteins of chaperonin containing TCP-1 and hypothetical protein XP_346548 were phosphorylated significantly. The proteins of 94 kD glucose-regulated protein, calpactin I heavy chain and ferritin were dephosphorylated markedly (P
