Isolation and differentiation of stem cells derived from human placenta into cardiomyocytes
- VernacularTitle:人胎盘来源的干细胞提取及其向心肌细胞分化的实验研究
- Author:
Yanqiu YU
;
Kun LI
;
Yunan JIN
;
Wei YUN
;
Lili DU
;
Xiaomei LU
;
Haiqin REN
- Publication Type:Journal Article
- Keywords:
Placenta;
Stem cells;
Cardiomyocytes;
Cell differentiation
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To isolate the stem cells from human placenta and induce them into cardiomyocytes. METHODS: Stem cells were isolated from human placenta and characterized by morphologic analysis. The “hanging drop” methods were used to inducte stem cells into cardiomyocytes. The expressions of atrial natriuretic factor(Anf) and ?-myosin heavy chain(?-MHC) genes were analyzed by RT-PCR. RESULTS: Stem cells derived from human placenta were self-renewed and differentiated into cardiomyocytes in vitro. RT-PCR showed that Anf and ?-MHC genes were expressed in the “beating cells”.CONCLUSION: Human placenta is an abundant source of stem cells.
