Effect of TMB-8 on the activation, proliferation and cell-cycle distribution of the mouse T lymphocytes in vitro
- VernacularTitle:TMB-8对小鼠T淋巴细胞体外活化、增殖和细胞周期的影响
- Author:
Xueyi YE
;
Yaoying ZENG
;
Xiuyan HUANG
;
Tong WANG
;
Ning ZANG
;
Jianguo ZHOU
;
Changle LIN
- Publication Type:Journal Article
- Keywords:
Trimethoxybenzoate;
T-lymphocytes;
CD69;
Cell proliferation;
Cell cycle;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of [8-(diethylamino) octyl-3, 4, 5 -trimethoxybenzoate] (TMB-8), an intracellular Ca2+ antagonist, on the activation, proliferation and cell-cycle distribution of the mouse T lymphocytes stimulated by concanvalin A (Con A) in vitro. METHODS: After stimulated with Con A, T cells were treated with different concentrations of TMB-8 alone and its combination with cyclosporine A (CsA). The expression of CD69, the early marker of CD3+ T cell activation, was measured by FACS. The proliferation-related index was determined by carboxyl fluorescin diacetate succinmidyl ester (CFDA-SE) flow cytometry. The cell-cycle distribution was analyzed by propidium iodide staining.RESULTS: After 6 h culture, the activation rate of CD69+ T cell in Con A group was (74.88?1.88)%. 10, 20 and 40 ?mol/L of TMB-8 inhibited the expression of CD69 (P
