p16 gene methylation and its protein expression in pancreatic carcinoma
- VernacularTitle:胰腺癌中p16基因甲基化改变及其蛋白表达分析
- Author:
Weihua YANG
;
Chunyou WANG
;
Qiushi ZHU
;
Lei YAN
;
Zhou XU
- Publication Type:Journal Article
- Keywords:
Pancreatic Neoplasms;
p16 Gene;
Methylation;
Methylation-specific PCR;
p16 Protein Expression
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate aberrant methylation in the promoter area of p16 gene and p16 protein expression in human pancreatic carcinoma and in the corresponding tumor-adjacent tissues,and evaluate their role in the carcinogenesis and progression of tumor and its clinical significance.Methods Immunohistochemistry and MSP(methylation-specific PCR)were performed on 46 samples of pancreatic carcinoma and their corresponding tumor-adjacent tissue specimens for p16 and its methylation.Results Expression rate of p16 protein was 41.3%(19/46)in pancreatic carcinomas,95.7%(44/46)in corresponding tumor-adiucent tissues.Through MSP,the methylation rate in pancreatic carcinomas was 39.1%.No gene methylation was found in 19 cases expressing p16 protein.p16 gene methylation was closely related to p16 protein expression in pancreatic carcinoma(P0.05);but were significantly related to the PTNM staging,histological differentiation,distant metastasis and lymph node metastasis(P
