Blocking the CC chemokine receptor 5 pathway by antisense peptide nucleic acid prolongs islet allograft survival
- VernacularTitle:反义肽核酸阻断CC趋化因子受体5途径延长同种异体胰岛移植物存活
- Author:
Lei YANG
;
Yongfeng LIU
;
Ying CHEN
;
Rui ZHANG
;
Dazhi FU
;
Tiemin LI
;
Ning ZHAO
- Publication Type:Journal Article
- Keywords:
Islet of Langerhans Transplantation;
CC Chemokine Receptor 5;
Peptide Nucleic Acid,Antisense;
Allograft Rejection
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the effect of peptide nucleic acid of CC chemokine receptor 5 on acute rejection of islet allograft.Methods Mice islet transplant models were used to test the effect of PNA CCR5 by targeting CCR5 in acute allograft rejection.In vitro T cell proliferative responses were assessed by mixed lymphocyte response(MLR).RT-PCR and Western blot were used to detect the expression of mRNA and protein.Results PNA CCR5-treated recipients demonstrated statistically significant prolongation(12.00?1.75)d in functional allograft survival when compared with saline(6.50?0.58)d or PNA mismatch-treated recipients(6.50?0.50)d.The CCR5 mRNA expression level of PNA CCR5,control,and PNA mismatch treatment recipients at day 7 posttransplant was 0.56?0.05,1.68?0.07 and 1.80?0.14,respectively.The data showed that CCR5 protein was significantly down-regulated in PNA CCR5 treatment allografts compared with saline and PNA mismatch treatment allografts(P
