Preparation of Mitomycin for Injection Nanoparticles by Microfluidization and Its Hemolytic Test
- VernacularTitle:微射流法制备注射用丝裂霉素纳米粒及其溶血试验研究
- Author:
Hongling ZHOU
;
Jianhai CHEN
;
Xiujuan AI
;
Xixiao YANG
;
Na XI
;
Qingfeng JIANG
- Publication Type:Journal Article
- Keywords:
Mitomycin;
Polybutylcyanoacrylate;
Nanoparticles;
Microfluidizer;
Hemolytic test
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To prepare Mitomycin C for injection-Polybutylcyanoacrylate Nanoparticles (MMC-PBCA-NPs) freeze drying preparation by microfluidization and to investigate its hemolytic feature. METHODS: The crude emulsion of MMC-PBCA-NPs was prepared by emulsion polymerization method, from which MMC-PBCA-NPs solution was prepared by microfluidization method; the encapsulation efficiency (EE) and the drug loading (DD) of the preparation were detected by ultraviolet spectrophotometry. The particle size and appearances of which were observed. A hemolytic test was performed on rabbits to observe the blood reaction of the preparation. RESULTS: The prepared MMC-PBCA-NPs freeze drying preparation was uniformly distributed and in round shape, with EE, DD and mean diameter at (85.1?3.8)%, (7.0?0.2)% and (113.5?3.86)nm, respectively. The hemolytic test was negative. CONCLUSION: It is feasible to prepare MMC-PBCA-NPs by microfluidization method.
