Experimental Study on Ectopic Allotransplantation of Rats Fetal Ovaries
- VernacularTitle:大鼠胚胎卵巢异体异位移植的实验研究
- Author:
Yunxiu LI
;
Xiaoming NIU
;
Xiaomei ZHANG
;
Yanping MA
;
Yonggang LI
- Publication Type:Journal Article
- Keywords:
Rats;
Fetal ovaries;
Allotransplantation
- From:
Journal of Kunming Medical University
1990;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the survival,growth and function restoration of fetal ovary allotransplantation.Methods 18~20 days fresh and frozen-thawed rat ovaries were ectopic allotransplanted subcutaneously to adult female SD rats that had been castrated for 4 weeks.Vaginal smears were taken daily after transplantation to observe the restoration of estrous cycles,and serum oestradiol(E_2) and progesterone(P) levels were measured at different time.Histological morphology of endometrium and ovaries were compared among four groups.Results 52.17%of the recipients in fresh group and 38.01% in frozen-thawed group resulted in restoration of regular estrous cyclic activity,and hormone level became normal on day 35 and day 49 after transplantation.The grafts grew up and follicles in different developing stage and corpora lutea could be observed under microscope.Endometrium had hormone effects.Conclusions This study indicates that subcutaneously allotransplanted fetal ovaries can survive,develop and restore the function of secreting sex hormone.The ultraspeed cooling rats fetal ovaries can be survival when they were thawed and develop and secrete hormone as fresh ovaries after being allotransplanted.
