Reduction of inflammatory-related factor expression in experimental acute pancreatitis in Egr-1 knockout mice
- VernacularTitle:Egr-1基因剔除减少炎性相关因子在实验性胰腺炎中的表达
- Author:
Youcai DUAN
;
Bo JIANG
;
Gaofeng MA
;
Zhimin XU
;
Xiaowen CHEN
;
Tianming CHENG
;
Yichen DAI
;
Xueqing CHEN
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
Early gowth response factor;
Genes,Egr-1;
Gene knock-out;
Chemotactic factors,macrophage
- From:
Chinese Journal of Pathophysiology
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the effects of Egr-1 gene knockout on the expression of inflammatory-related factors in pancreatic tissue in a mouse acute pancreatitis model.METHODS: The experimental pancreatitis was induced by high-dose of cearulein in wildtype mice and Egr-1 knockout mice.The pancreatitis indexes,such as serum amylase,pancreata edema,and myeloperoxidase(MPO) levels in pancreata and lungs were recorded.The mRNA levels of tissue factor(TF),plasminogen activator inhibitor(PAI-1),monocyte chemoattractant protein(MCP-1),Gro-1,IL-6 and ICAM-1 were measured by quantitative PCR.RESULTS: Contrary to wildtype mice,typical pancreatitis was not induced by high-dose cearulein in the Egr-1 knockout mice,not only markedly reduced edema in pancreata and lungs,but decreased MPO levels in lungs as well were found.Furthermore,the mRNA of TF,PAI,MCAP,ICAM-1 and IL-6 in pancreata were significantly decreased in Egr-1 knockout mice.CONCLUSION: The severity of pancreatitis and lung damage is ameliorated in Egr-1 knockout mice stimulated by high-dosage of cearulein,which was probably mediated by decreasing expression of inflammatory-related factors in pancreata,such as TF,PAI,MCP-1,ICAM-1 and IL-6.
