Inducing Rabbits Bone Mesenchymal Stem Cells into Chondrocytes in Vitro
- VernacularTitle:兔骨髓基质干细胞体外定向分化为软骨细胞
- Author:
Linsong JI
;
Di WU
;
Jian DONG
;
Shihe LI
;
Shaofeng TANG
;
Tao MA
- Publication Type:Journal Article
- Keywords:
Bone mesenchymal stem cell;
Chondrocyte;
Induction
- From:
Journal of Kunming Medical University
2007;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce bone marrow mesenchymal stem cells(BMSCs) or bone marrow stroma stem cell(MSCs) to differentiate directionally towards chondrocytes in vitro and then identify the differentiated cells.Methods Bone marrow was harvested from the iliac bone of 16-week-old Japanese white rabbits.After gradient centrifugation,cultivation,amplification,the 3rd-passaged BMSC were implanted in six-hole-plate according to a certain proportion and induced by chondrogenic inducers including transforming growth factor-?1,dexamethasone and vitamin C.Chondrocytes were selected and fixed at different time.The features of chondrocytes were identified by toluidine blue staining and collagen types II immunohistochemical assay.Results The structure of cellular cartilage from BMSCs was uniformly positive of toluidine blue staining and collagen types II immunohistochemical staining.Conclusion Rabbit BMSC,obtained under the experimental conditions,develops stably,proliferates rapidly and is differentiated successfully into chondrocytes by induction in vitro.
