Studies on Preparation and Physicochemical Properties of Recombined Human Interferon-? Microspheres
- VernacularTitle:基因重组人干扰素-?微球的制备及理化性质研究
- Author:
Fenglan SONG
;
Fan YANG
;
Yiqun YANG
;
Xingchen LIU
- Publication Type:Journal Article
- Keywords:
Interferon-?;
Poly(lactic acid-co-glycolic);
Microspheres;
Preparation;
Physicochemical properties
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare the gene recombined human interferon-alpha poly(lactic acid-co-glycolic)microspheres and study its physicochemical properties.METHODS:Interferon-? microspheres were prepared by W/O/W solvent extraction technique;the in vitro release of microspheres were examined;the rudimental organic solvent was determined with GC-MS.RESULTS:The IFN-?-MS were regular in their morphology,and the particle size distribution was narrow.The average diameter was 45.54?m.The encapsulation rate and the loading efficiency were 83.49% and 8.03%,and the in vitro accumulative release rate was 80.32% in 30 days.The in vitro interferon-? release from the microspheres was best described using Higuchi diffusion model.The rudimental of dichloromethane was less than 0.05%.CONCLUSIONS:The prepared IFN-?-MS is very good in all aspects of its physicochemical properties.
