Content Determination of Levodopa in Levodopa Inclusion Complex by HPLC
- VernacularTitle:HPLC法测定左旋多巴包合物中主药含量
- Author:
Lisa XU
;
Yong SUN
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Levodopa;
Inclusion complex;
Content determination
- From:
China Pharmacy
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of levodopa in levodopa inclusion complex.METHODS:The separation was performed on C18,and the mobile phase consisted of methanol-0.01mol?L-1 potassium dihydrogen phosphate buffer solution(25∶75,pH 3.0)with a flow rate of 1.0mL?min-1.The column temperature was 30℃,the detection wavelength was 280 nm and the sample size was 20?L.RESULTS:A good linear relationship was obtained for levodopa within the range of 5～80?g?mL-1(r=0.999 8),and its average recovery was 100.1%(RSD=0.19%,n=15).CONCLUSION:This method is simple,rapid,accurate,and it can be used for the content determination of levodopa in levodopa inclusion complex.
