Determination of the Contents of Quercetin and Isorhamnetin in Bupleurum. smithii var. parvifolium Shan et Y. Li by HPLC
- VernacularTitle:高效液相色谱法测定小叶黑柴胡中槲皮素与异鼠李素的含量
- Author:
Wei ZHOU
;
Guangming CAI
;
Hehui HUANG
;
Ehu LIU
;
Haisheng ZHU
;
Hanhui XIONG
- Publication Type:Journal Article
- Keywords:
HPLC;
Bupleurum.smithii var.parvifolium Shan et Y.Li;
Quercetin;
Isorhamnetin;
Content determination
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish an HPLC method for determining the contents of Quercetin and Isorhamnetin in Bupleurum.smithii var.parvifolium Shan et Y.Li.METHODS:The HPLC was performed on Kromasil column C18(250mm? 4.6mm,5? m),using methanol-0.4% phosphoric acid(55∶ 45)as mobile phase,with flow rate at 1.0mL? min-1 and detection wavelength at 256nm.RESULTS:The linear range of Quercetin was 0.08～ 0.40? g(r=0.999 6),with an average recovery rate of 101.02%(RSD=1.53%);that of Isorhamnetin was 0.06～ 0.30? g(r=0.999 2),with an average recovery rate of 101.26%(RSD=2.95%).CONCLUSION:This method is simple and accurate with good reproducibility.It is suitable for the quality control of Bupleurum.smithii var.parvifolium Shan et Y.Li.
