RNA interference inhibits the secretion of IL-1? in mice spleen lymphocytes
- VernacularTitle:RNA干扰抑制小鼠脾淋巴细胞分泌IL -1?
- Author:
Ningning ZHENG
;
Yanqiu YU
;
Xiaoyu SONG
;
Xudong DING
;
Haipeng ZHANG
- Publication Type:Journal Article
- Keywords:
RNA interference;
Post-transcriptional gene silencing;
Interleukin-1;
Lymphocytes
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To decide the effect that selected siRNA degrades mRNA of IL-1? specifically and suppression of its expression after connected with target site with homology complementary sequence. METHODS: Synthesized DNA expression box aimed directly at target site through PCR reaction in vivo was purified, and transfected into lymphocytes stimulated by LPS. siRNA was transcribed by cellular endogenous RNA polymerase Ⅲ and then evoke the degradation of target mRNA. After 48 hours of transfection, the cell culture supernatant was collected and the concentration of IL-1? was assayed using ELISA. RESULTS: Compared with blank-control and negative-control, selected sequence decreased the expression of IL-1?. Rate of the suppression was about 15%. CONCLUSION: RNAi technology produces specific interference effect in mouse spleen lymphocytes in original culture and inhibits the excretion of IL-1?.
