Inhibitory effect of sodium ferulate on A?_(25-35)-induced p38 MAPK activation
- VernacularTitle:阿魏酸钠抑制A?_(25-35)诱导的小鼠腹腔巨噬细胞p38MAPK活化
- Author:
Suyan YAO
;
Deyu ZHENG
;
Shuyun LI
;
Jinfa ZOU
;
Ying JIN
- Publication Type:Journal Article
- Keywords:
Sodium ferulate;
Amyloid beta-protein;
Mitogen-activated protein kinases;
Macrophages.
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate effect of sodium ferulate on A?25-35-mediated signaling pathway. METHODS: The isolated peritoneal macrophages from mice were cultured. p38 MAPK protein kinase in nuclear extracts was analyzed by Western blotting. The concentration of TNF-? and NO in supernatant were measured by ELISA and Griess reaction technique. The expression of iNOS protein was detected by immunochemical technique. RESULTS: A?25-35 significantly increased the concentrations of TNF-? and NO in supernatant, expression of iNOS in macrophages and p38 MAPK protein kinase in nuclear extracts, which were blocked by sodium ferulate. CONCLUSION: Sodium ferulate inhibits p38 MAPK activation triggered by A?25-35.
