Effects of FKBP12.6 gene on intracellular Ca~(2+) concentration in mouse H9c2 (2-1) myocardial cells transfected by ultrasound-mediated destruction of microbubbles
- VernacularTitle:超声微泡介导转染FKBP12.6基因对小鼠H9c2(2-1)心肌细胞中Ca~(2+)浓度的影响
- Author:
Guotong LIU
;
Chengming YANG
;
Xukai WANG
;
Chunyu ZENG
;
Hongyong WANG
;
Yuqiang FANG
;
Chunjiang FU
;
Weibin SHI
- Publication Type:Journal Article
- Keywords:
Ultrasound;
Microbubble agent;
Gene transfection;
H9c2(2-1) cells
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the changes of intracellular calcium ion (Ca2+) concentration in mouse H9c2 (2-1) cells transfected with or without FK506 binding protein 12.6(FKBP12.6) gene by ultrasound mediated destruction of microbubbles. METHODS: The pcDNA3.1-FKBP12.6 plasmid, mingled with albumin-coated microbubbles agents, was transfected into H9c2 (2-1) cells by ultrasound-mediated destruction of microbubbles. The H9c2 (2-1) cell growth state was investigated by inverted microscope. The changes of intracellular Ca2+ concentration was determined by laser scanning confocal microscope. The FKBP12.6 protein expression was checked by immunohistochemistry. RESULTS: As compared with control cells, the H9c2 (2-1) cells, transfected with FKBP12.6 gene, grew better, had higher gross intracellular Ca2+ concentration. CONCLUSION: FKBP12.6 gene augments Ca2+ concentration in mouse H9c2 (2-1) cells, enhances the contractibility of the myocardial cell, which may be helpful to improve the myocardial dysfunction.
