Role of endothelin in portal hypertension induced by endotoxin
- VernacularTitle:内皮素-1在脂多糖致大鼠门脉高压症中作用的体外研究
- Author:
Xiangjun BI
;
Minhu CHEN
;
Wenhong CHEN
;
Wei CHEN
;
Jinhui WANG
;
Senlin ZHU
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Endothelin-1;
Hypertension, portal;
Oligonucleotides, antisense;
Hepatic stellate cells
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the role of endothelin-1 (ET-1) in portal hypertension (PHT) induced by endotoxin. METHODS: Collagenase in situ perfusion was adopted to separate hepatic stellate cells (HSCs). HSCs was cultured on concretized collagen. ET-1 anti-sense oligonucleotide was added into the culture medium and then LPS was also added up to the concentration of 1 000 ?g/L. The diameters of the concretized collagen were measured. Sense and mis-sense oligonucleotide were applied as control. ET-1 in the culture medium was detected by radioimmunoassay and ET-1 mRNA in HSCs was detected by RT-PCR. ?-actin of HSCs was detected by Western blotting. RESULTS: The diameter of concretized collagen on which HSCs pretreated with ET-1 anti-sense oligonucleotide was 93.3%?3.8% the size of the primary. The diameter of concretized collagen of the control groups were 70.1%?4.8% and 70.5%?3.9% (P
