Fatty acid-induced insulin resistance in 3T3-L1 adipocytes
- VernacularTitle:脂肪酸诱导的3T3-L1脂肪细胞胰岛素抵抗的研究
- Author:
Yu WEN
;
Hongwei WANG
;
Huiling LU
;
Xiufen HU
;
Hong SHI
;
Yang YANG
;
Min KANG
;
Dandan WANG
- Publication Type:Journal Article
- Keywords:
Adipocytes;
Insulin;
Fatty acids;
Glucose transport;
Insulin resistance
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The purpose of the present study was to examine the effect of insulin and different kinds of free fatty acid (FFA) on glucose transport in cultured 3T3-L1 preadipocytes or adipocytes. METHODS: Following the exposure of preadipocytes to insulin at different concentrations and treated times, glucose transport was assessed as [3H]2-deoxy glucose uptake. Furthermore, 3T3-L1 preadipocytes and adipocytes with either the monounsaturated FFA oleate (C18:1) or the saturated FFA palmitate (C16:0)were used and glucose transport was examined as above. RESULTS: Insulin increased specific membrane glucose transport in 3T3-L1 preadipocytes at the time of 15 min to 1 h stimulation. However, after 6 h exposure to insulin, downregulation of glucose transport was observed. Dose response studies demonstrated that 2-DG transport increased by 336% at 50 nmol/L of insulin (P
