Survival and distribution of CD34~+/CD45~+ cells from bone marrow of transgenic GFP mouse in a completely transected spinal cord rat model
- VernacularTitle:绿色荧光蛋白转基因小鼠CD34~+/CD45~+细胞在脊髓全横断大鼠模型中的存活及迁移
- Author:
Haoyu CHENG
;
Mingsheng HE
;
Tinghua WANG
;
Hongtian ZHANG
;
Xicai WANG
;
Zhiping WU
;
Fangfang WANG
;
Kun LIU
;
Guiqin HUANG
;
Yanli HU
- Publication Type:Journal Article
- Keywords:
CD34+/CD45+ cells;
Spinal cord, complete transected;
Green fluorescent protein;
Cell movement;
Rats
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the survival, transfer and distribution of bone marrow CD34+/CD45+ cells from transgenic GFP mouse after transplanted into the completed transversional spinal cord rat model. METHODS: The bone marrow cells isolated from transgenic GFP mice were cultured in vitro. The cultured cells were identified by anti-CD34 and anti-CD45 monoclonal antibodies, and were transferred into the end of transection spinal cord. Paraformaldehyde was infused into the left ventricle of the rat model at the 24 h, 48 h, 1 week, 2 weeks, 4 weeks and 8 weeks after cell transplantation. Through sank and frozen, the spinal cord was sectioned at 10 ?m thickness. The green fluorescence positive cells were observed under the fluorescence microscope. CD34+/CD45+ cells were identified by immunohistochemistry staining. RESULTS: Green fluorescence positive cells were found at the head and the end of the completed transection part of spinal cord. Most of the green fluorescence positive cells were distributed in the gray substance of spinal cord. CD34+/CD45+ cells were found by immunohistochemistry staining. CONCLUSION: CD34+/CD45+ cells survived in spinal cord of SD rat, and migrated to the head of the transection part. The distance of migration was extended by the time.
