Determination of Dexamethasone Acetate in Compound Tretinoin Ointment by RP-HPLC
- VernacularTitle:RP-HPLC法测定复方维A酸软膏中醋酸地塞米松的含量
- Author:
Ling YAN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Compound tretinoin ointment;
Dexamethasone acetate;
Content
- From:
China Pharmacy
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To develop a method for determination of the content of Dexamethasone acetate in Compound tretinoin ointment by RP-HPLC. METHODS: The separation was performed on C18 column with methanol: water (70∶30) as a mobile phase, wavelength of UV detector at 240nm. RESULTS: The linear range of Dexamethasone acetate was within 0.028 3～0.849 0?g(r=0.999 9). The average recovery of Dexamethasone acetate was 101.13%, with RSD at 1.28%. CONCLUSION: The method is simple, rapid and capable of producing good separation results. It can be applied to the quality control of Compound tretinoin ointment.
