Determination of Diosgenin in Di’ ao Xinxuekang Capsule by HPLC
- VernacularTitle:高效液相色谱法测定地奥心血康胶囊中薯蓣皂苷元的含量
- Author:
Zhuo LI
- Publication Type:Journal Article
- Keywords:
Di’ ao Xinxuekang Capsule;
Diosgenin;
HPLC;
Assaying
- From:
China Pharmacy
1991;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the method for the determination of diosgenin in Di’ ao Xinxuekang Capsule by HP_LC. METHOD: The condition of HPLC was Shim-pack CLC-Sil columm ( 150mm? 6. 0mm) , mobile phase of 2. 0% isopropand in petroleum ether and UV detector at 206nm. RESULTS : The method proved to be linear in the range at 0. 58～ 11. 6? g of diosgenin with correlation coefficient of 0. 999 8. The linear formula was Y=3. 682? 107X+ 1700, The average recovery and recision were 99. 49% , and RSD=0. 12% ( n=5) . CONCLUSION: The method is simple, accurate, specific and can be used to the quality control of the preparation.
