Preparation of rat tail collagen and a cell culture model of human nasal epithelial cells
- VernacularTitle:鼠尾胶原为底物的人鼻腔纤毛上皮细胞培养模式的建立
- Author:
Xiaohong SONG
;
Luo ZHANG
;
Demin HAN
;
Erzhong FAN
;
Hong WANG
;
Kuiji WANG
;
Ying LI
- Publication Type:Journal Article
- Keywords:
Humans;
Nasal Cavity;
Epithelial Cells;
Cell Culture Techniques;
rat tail collagen
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a cell culture of human nasal epithelial cells on glass cover slides coated with rat tail collagen and explore a method to research human mucociliary system. METHODS The rat tail collagen and collagen coated cover slides were prepared for explant culture of human nasal epithelial cells. RESULTS The collagen on cover slides must be thin and ? at. It was about 1mm thick. The cultured epithelial cells were monolayer and polygonal cells. There was tight junction between the cells. Ciliary beat frequency of different cilia was equal on the same cell,and it was also equal of on cells from uncinate process and inferior turbinate. CONCLUSION This successful cell culture model of human nasal epithelail cell could be helpful to research the physiology and function of human nasal mucociliary system.
