Effect of liposome-mediated transfection of Kv1.5 antisense oligonucleotides on activity of Kv in airway smooth muscle cells
- VernacularTitle:脂质体转染Kv1.5反义寡核苷酸(AsOND)对人气道平滑肌Kv活性的影响
- Author:
Dongjun CHENG
;
Yongjian XU
;
Xiansheng LIU
;
Shengdao XIONG
;
Zhenxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Potassium channels;
Airway smooth muscle cells;
Oligonucleotides,antisense;
Transfection
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the activity change of voltage-dependent delayed rectifier potassium channel(Kv) on human airway smooth muscle cells(HASMCs) after transfection of Kv1.5 antisense oligonucleotides(AsOND),and to discuss the regulating mechanism of Kv1.5.METHODS: The mRNA and protein expressions of Kv1.5 in liposome-mediated Kv1.5 AsOND transfected HASMCs were measured with techniques of reverse transcriptase-polymerase chain reaction(RT-PCR) and Western blotting.Kv activities in transfected HASMCs were investigated with whole-cell patch clamp.RESULTS: After HASMC were transfected by liposome-mediated Kv1.5 AsOND,the mRNA and protein expressions of Kv1.5 were decreased,and Kv activity was inhibited,which made the cell membrane potential(Em) inclined to depolarization.CONCLUSION: Transfection of Kv1.5 AsOND made the function of Kv in HASMCs decreased.Kv1.5 may play a critical role in the regulation of Kv activity.
