Effect of sodium nitroprusside on apoptosis in human airway smooth muscle cells of passive sensitization by serum from allergic asthmatic patients
- VernacularTitle:硝普钠诱导被动致敏的人气道平滑肌细胞凋亡
- Author:
Tao YE
;
Yongjian XU
;
Zhenxiang ZHANG
;
Xiansheng LIU
;
Yaqing LI
;
Yijun TANG
;
Sheng ZHONG
- Publication Type:Journal Article
- Keywords:
Nitric oxide;
Passive sensitization;
Apoptosis;
Muscle,smooth;
Airway
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate NO-donor sodium nitroprusside(SNP)-induced apoptosis of human airway smooth muscle cells(HASMCs) of passive sensitization by serum from allergic asthmatic patients.METHODS: The technique of human airway smooth muscle(HASMCs) passively sensitized with serum from allergic asthmatic patients was adopted.The effect of SNP on the survival rate of passively sensitized HASMCs was detected by MTT method.Apoptosis of cells was detected by TUNEL and flow cytometry.RESULTS:(1) Compared to sensitized group,the survival rate of passively sensitized HASMCs decreased in SNP+sensitized group(n=5,P
