Experimental study of antitumor immunity of DC/C6 fusion vaccine
- VernacularTitle:DC/C6融合瘤苗防治C6胶质瘤的实验研究
- Author:
Donghai WANG
;
Xingang LI
;
Ying WANG
;
Xun QU
;
Gang LI
;
Songfeng GONG
;
Quanmeng LIU
;
Xiufeng BAO
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Glioma;
Vaccines;
Immunotherapy
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the anti-tumor effect and mechanism of fusion vaccine on DCs and C6 glioma cells.METHODS: PEG was used to fuse DCs with C6 glioma cells.Immunofluorescence with GFAP-FITC was used to identify the DC/C6 fusion cells.Rat brain glioma models were made by stereotactic technique.After 5 days of inoculation of C6,107 fusion cells were injected through tail vein in group A.The same number of DCs and the same volume of PBS were used in group B and group C.The survival time of rats in these three groups was analyzed by Log-rank survival analysis.Tumor samples were checked by HE staining and immunohistochemical staining with CD8Mcab.RESULTS: Positive result of GFAP-FITC immunofluorescence was observed in DC/C6 fusion cells.The Log-rank survival analysis showed that statistically significant difference in group A was observed compared to that in group B and group C(P
