Effects of angiotensin Ⅱ on ATP binding cassette transporter A1 in THP-1 derived foam cells
- VernacularTitle:血管紧张素Ⅱ对THP-1源性泡沫细胞ATP结合盒转运子A1的影响
- Author:
Zhijian CHEN
;
Yanfu WANG
;
Yuhua LIAO
;
Chunli MEI
;
Hongyu PENG
;
Heping GUO
- Publication Type:Journal Article
- Keywords:
Angiotensin II;
ATP-binding cassette transporters;
Arteriosclerosis;
Foam cells
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To study the influence of angiotensin Ⅱ(AngⅡ) on ATP-binding cassette transporter A1 in THP-1 derived foam cells.The variance of the expression of ABCA1,the content and the effluent rate of cholesterol were also investigated.METHODS: The regulatory effect of AngⅡ on the expression of ABCA1 mRNA and protein in THP-1 derived form cells were measured by RT-PCR and Western blotting.The effect of variance of cholesterol content was measured by zymochemistry via-fluorospectrophotometer,cholesterol effluent was measured by liquid scintillator.RESULTS: A positive facilitative effect of Ang Ⅱon form cells was observed.Total cholesterol content were increased significantly by Ang Ⅱ treatment(P
