Determination of Schisandrin and Schisandrin B in Compound Wurenchun Capsule by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定复方五仁醇胶囊中2组分的含量
- Author:
Zhihua DOU
;
Anwei DING
;
Beihua BAO
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Compound wurenchun capsule;
Schisandrin;
Schisandrin B;
Assaying
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of schisandrin and schisandrin B in compound wurenchun capsule by RP-HPLC.METHODS:Column lichrosphere C18 was used,mobile phase of water and MeOH(gradient elution)was set up,the flow rate was 1.0mL?min-1,the column temperature was 30℃and the detection wavelength was 254nm.RESULTS:The linear ranges of schizandrin and schisandrin B were within 0.182 4～1.641 6?g(r=1.000 3)and 0.189 6～ 1.706 4?g(r=0 .999 9),respectively.The average recoveries were 98.32%(RSD=1.02%)and 97.22 %(RSD=0.94%),respectively.CONCLUSION:The established method is convenient,accurate and highly specific,and it can be used for the quality control of compound wurenchun capsule.
