Determination of Baicalin and Jasminoidin in Qingkailing Injection by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定清开灵注射液中2组分的含量
- Author:
Xuyuan ZHAO
;
Ling HU
;
Xiaolu HAN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Qingkailing injection;
Baicalin;
Jasminoidin;
Assaying
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of baicalin and jasminoidin in Qingkailing Injection.METHODS:It was performed on DiamonsilTM C18 column with acetonitrile-20 mmol?L-1 sodium dihydrogen phosphate solution (20:80)as mobile phase.The flow rate was 1.0 mL?min-1.The detection wavelength was 238 nm.RESULTS:The liner range of baicalin was 1～50?g?mL-1,(r=0.999 5)and the average recovery was 100.75%(RSD=1.75%);The liner range of jasminoidin was 0.1～10?g?mL-1(r=0.999 7)and the average recovery was 100.63%(RSD=1.35%).CONCLUSION:This method is rapid,simple,accurate and sensitive.It can be used for quality control of Qingkailing Injection.
