Determination of Sarsasapogenin in Jujube Seed Concentrated Pills by RP-HPLC-ELSD
- VernacularTitle:反相高效液相色谱-蒸发光散射检测法测定酸枣仁浓缩丸中菝葜皂苷元的含量
- Author:
Xudong CHENG
;
Xiaobin JIA
;
Yan CHEN
;
Yin CAI
- Publication Type:Journal Article
- Keywords:
Jujube seed concentrated pills;
Sarsasapogenin;
RP-HPLC-ELSD;
Assaying
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine Sarsasapogenin in Jujube seed concentrated pills by RP-HPLC-ELSD.METHODS:Separation of Sarsasapogenin was performed on Zorbax C18 column with methanol-water (90:10)as a mobile phase at a flow rate of 1.0mL?min-1.The temperature of the drift tube was 85℃and the air flow-rate was at 1.71mL?min-1.RESULTS:The linear range of Sarsasapogenin was 0.112 7～0.676 2mg?mL-1(r=0.998 3).The average recovery was 99.83%(RSD=0.93%).CONCLUSION:The method is simple,accurate and reproducible,and suitable for the quality control of Jujube seed concentrated pills.
