Determination of Notoginsenoside R_1,Ginsenoside Rg_1 and Rb_1 in Sanqishangyao Tablets by HPLC
- VernacularTitle:高效液相色谱法测定三七伤药片中3组分的含量
- Author:
Yuduan YAO
;
Ping JIANG
;
Wenzhong ZHANG
- Publication Type:Journal Article
- Keywords:
Sanqishangyao Tablets;
Notoginsenoside R_1;
Ginsenoside Rg_1;
Ginsenoside Rb_1;
HPLC;
Assaying
- From:
China Pharmacy
1991;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To set up a HPLC method for the determination of the contents of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao tablets.METHODS:It was performed on VP-ODS (150mm?4.6mm,5?m),and the mobile phase was acetonitrile-water with gradient elution system(0～20min(20∶80),20～26min(40∶60),26～35min(20∶80)).The flow rate was 1.0mL?min-1 and the column temperature was room temperature.The detection wavelength was 203nm.RESULTS:The liner range of notoginsenoside R1 was 0.261～7.83?g(r=0.999 9)and the average recovery was (101.12?2.03)%(RSD=2.1%).The liner range of ginsenoside Rg1 was 0.602 5～18.075?g(r=0.999 7)and the average recovery rate was (97.22?3.33)%(RSD=1.9%).The liner range of ginsenoside Rb1 was 0.537 5～16.125?g(r=0.999 6)and the average recovery rate was(100.71?2.4)%(RSD=1.6%).CONCLUSION:The method is suitable for the content determination of notoginsenoside R1,ginsenoside Rg1 and Rb1 in Sanqishangyao Tablets.
