Transfection of human Kv1.5 gene inhibits the proliferation of human airway smooth muscle cells
- VernacularTitle:转染Kv1.5基因抑制人气道平滑肌细胞的增殖
- Author:
Limin ZHAO
;
Yongjian XU
;
Shengdao XIONG
;
Zhenxiang ZHANG
- Publication Type:Journal Article
- Keywords:
Gene transfection;
Airway smooth muscle cells;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of human Kv1.5 gene transfection on the proliferation and apoptosis in human airway smooth muscle cells(HASMCs).METHODS:Kv1.5 gene was transiently transfected into HASMCs with Lipofectamine 2000 and the level of Kv1.5 protein was observed by Western blotting.Ca 2+ concentration in HASMCs was investigated by fluorescent quantitation using fluorospectrophotometer.Flow-cytometry,MTT method and TUNEL were used to detect the effects of Kv1.5 gene transfection on proliferation and apoptosis in HASMCs.RESULTS:(1)Western blotting showed that transfection of human Kv1.5 plasmid significantly increased the Kv1.5 protein expression compared to control group(P
