Determination of Hydroxy Safflor Yellow A in Orthopaedics Lotion 1 by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定骨科洗剂1号方中羟基红花黄色素A的含量
- Author:
Weiwen PENG
;
Yujiao GAO
;
Quanxi MEI
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Orthopaedics lotion 1;
Hydroxy safflor yellow A;
Content
- From:
China Pharmacy
2005;0(24):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of hydroxy safflor yellow A in Orthopae_dics lotion 1.METHODS:The separation was performed on Phenomenex Luna C18 with mobile phase composed of methanol-acetonitrile-water(26∶ 2∶ 72).The flow rate was 1.0ml/min;the detective wavelength was 403nm and the column temperature was 30℃.RESULTS:At a sample size of 0.1 044? g～ 1.2 528? g(r=0.9 998),hydroxy safflor yellow A was noted to be of good linear relation with peak area score.The average recovery was 98.08%(RSD=0.52%).CONCLUSION:The method is simple,accurate,specific,sensitive,reproducible,and suitable for the quality control of Orthopaedics lotion 1.
