Experimental Study on the Corneal Endothelial Cells Substituted by the Vascular Endothelial Cells(VEC) Cultivated on the Amniotic Membrane as its Growth Carrier
- VernacularTitle:羊膜作为载体培养血管内皮细胞替代自体角膜内皮细胞的研究
- Author:
Weibin YI
;
Zhulin HU
;
Yanze XU
;
Tinghua WANG
;
Zhewen LUO
;
Guiqin HUANG
- Publication Type:Journal Article
- Keywords:
Vascular endothelial cell;
Cell culture;
Autogenous corneal endothelium;
Amniotic membrane
- From:
Journal of Kunming Medical University
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Amniotic membrane as the carrier to culture the vascular endothelial cells was investigated in this study in order to explore whether the corneal endothelial cells superseded by the vascular endothelial cells is feasible on the account of directing a kind of the original method to handle the bullous keratopathy.Methods The vascular endothelial cells adhering to the sphagitides lumen of the experimental rabbits were digested and gained by means of the perfusion method with 0.25%tripsin plus 0.02%EDTA.Fresh amniotic membrane without the amniotic epithelial cells was cut into the square tissue about 1.5 cm?1.5 cm and spread evenly in the 24-well culture dish.Primary cultured cells were subcultured on the amniotic membrane.We would not transplant the vascular endothelial cells feeding on the amniotic membrane until cells is full of the whole amniotic membrane surface.One to two months after the operation,the change of corneal diaphaneity was observed.Results About 12 days since the cell transplantation was performed,the corneal transparency alteration between the experimental groups and the control one is different.The corneal buttons in the experimental group show the severe edema and opacity,and the anterior chamber couldn't be seen unclearly.But,10 days after the operation,the corneal oedema which begins to extenuate was judged through the indicatrix that the corneal edema in the experimental group has been recovering slowly,among which the anterior chamber tissue of 7 animals was visible through the implant.The corneal edema in the control groups intensified evidently,even,the part of these appeared the ulcerous necrosis as result of the corneal severely edema.There is the existence of difference between two groups(P
